\(\textit{n}\)-alkanol stress-induced cell envelope injury of \(σ^{E}\) promoter in \(\textit{Escherichia coli}\)

Authors

  • Huong Thi Bui Vietnam Institute of Agricultural Engineering and Post Harvest Technology, Vietnam; Kansai University, Suita, Osaka 564-8680, Japan; Nhat Lan Green Biotechnology Company, Ha Noi, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/17136

Keywords:

n-alkanols, membrane injury, envelope stress response, sigma E, Escherichia coli.

Abstract

To characterize the cellular stress by n-alkanols with different alkyl chain lengths in Escherichia coli, we investigated how n-alkanols damage cell envelope permeability and whether they enhance the promoter activity of the envelope stress response regulator, σE, by using variants of green fluorescent protein (GFP). By using E. coli cells having GFPuv expressing and localizing in the cytoplasm, the inner membrane, and the periplasm, after exposure to n-alkanols, the fluorescent intensity of GFPuv released from cells was examined. Our data showed that at the similar levels of cell death of about 90–97%, ethanol, a short-chain alkanol, at a concentration of 20% damaged the outer membrane more greatly than the inner membrane, whereas a longer-chain alkanol of pentanol at a concentration of 1.125% damaged both of the outer and inner membranes. Then we investigated the envelope stress response to n-alkanols by σE factor by ratiometric analysis of rpoE promoter activity for the downstream GFPuv expression referenced to that of housekeeping sigma 70 (σ70 ) recognizing lacUV5 promoter for red fluorescent protein (RFP) expression. The results indicated that the relative activity of rpoE promoter by pentanol was much greater than that of ethanol. The degree of its sensitization by rpoE deficiency was much more remarkable for cells treated with pentanol than for those with ethanol. The results suggest that the response of the σE plays a significant role in the membrane integrity and survival of E. coli cells treated with n-alkanols depending on the alkyl chain length of the molecule.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Tsuchido T., Katsui N., Takeuchi A., Takano M., Shibasaki I., 1985. Destruction of the outer membrane permeability barrier of Escherichia coli by heat treatment. Applied and Environmental Microbiology, 50(2): 298–303.

Ingram O. L., Vereeland S. N., 1980. Different Effects of Ethanol and Hexanol on the Escherichia coli cell envelope. Journal of Bacteriology, 144(2): 481−488.

Fried V. A., Novick A., 1973. Organic solvents as probes for the structure and function of the bacterial membrane: effects of ethanol on the wild type and an ethanol-resistant mutant of Escherichia coli K-12. J. Bacteriol, 114: 239−248.

Harold F. M., 1970. Antimicrobial agents and membrane function. Adv.Microb. Physio, 4: 45−104.

Hugo W. B., 1967. The mode of action of antibacterial agents. J. Appl. Bacteriol; 30: 17−50.

Lee A.G., 1976. Interaction between anesthetics and lipid mixtures: normal alcohols. Biochemistry, 15: 2448−2454.

Jain M. K., Wu N. M., 1977. Effect of small molecules on the dipalmitoyl lecithin liposomal layer. III. Phase transition in the lipid bilayer. J. Membr. Biol., 34: 157−201.

MacDonald A. G., 1978. A dilatometric investigation of the effects of general anesthetics, alcohols, and hydrostatic pressure on the phase transition in smectic mesophases of dipalmitoyl phosphatidylcholine. Biochim. Biophys. Acta, 507: 26−27.

Paterson S. J., Butler K.W., Huang P., Labelle J., Smith I. C. P., Scheneider H., 1972. The effects of alcohols on lipid bilayers: a spin label study. Biochim. Biophys. Acta, 266: 597−602.

Silbert D. F., 1970. Arrangement of fatty acids groups in phosphatidylethanolamine from fatty acid auxotroph of Escherichia coli; Biochemistry, 9: 3631−3640.

Sullivan K. H., Hegeman G. D., Cordes E. H., 1979. Alteration of the fatty acid composition of Escherichia coli by growth in the presence of normal alcohols. J. Bacteriol., 138: 133−138.

Ingram L. O., 1976. Adaptation of membrane lipids to alcohols. Journal of Bacteriology, 125(2): 670−678.

Hubbel W.L., Metcalfe J. C., Metcalfe S. M., McConnell H. M., 1970. The interaction of small molecules with spin - labeled erythrocyte membrane-active agents. Biochim. Biophys. Acta, 219: 415−427. doi: 10.1016/0005-2736(70)90219-1

Paterson S. J., Butler K. W., Huang P., Labelle J., Smith I. C. P., Schneider H., 1972. The effects of alcohols on lipid bilayers: a spin-label study. Biochim. Biophys. Acta; 266: 597−602.

Grisham C. M; Barnett R. E., 1973. The effects of long-chain alcohols on membrane lipids and the (Na+ + K+ )-ATPase. Biochim. Biophys. Acta, 311: 417−422.

Downloads

Published

23-06-2022

How to Cite

Bui, H. T. (2022). \(\textit{n}\)-alkanol stress-induced cell envelope injury of \(σ^{E}\) promoter in \(\textit{Escherichia coli}\). Academia Journal of Biology, 44(2), 91–104. https://doi.org/10.15625/2615-9023/17136

Issue

Section

Articles