@article{Bui_2022, title={\(\textit{n}\)-alkanol stress-induced cell envelope injury of \(σ^{E}\) promoter in \(\textit{Escherichia coli}\)}, volume={44}, url={https://vjst.net/index.php/vjbio/article/view/17136}, DOI={10.15625/2615-9023/17136}, abstractNote={<p>To characterize the cellular stress by <em>n</em>-alkanols with different alkyl chain lengths in <em>Escherichia coli</em>, we investigated how <em>n</em>-alkanols damage cell envelope permeability and whether they enhance the promoter activity of the envelope stress response regulator, σ<sup>E</sup>, by using variants of green fluorescent protein (GFP). By using <em>E. coli </em>cells having GFPuv expressing and localizing in the cytoplasm, the inner membrane, and the periplasm, after exposure to <em>n</em>-alkanols, the fluorescent intensity of GFPuv released from cells was examined. Our data showed that at the similar levels of cell death of about 90–97%, ethanol, a short-chain alkanol, at a concentration of 20% damaged the outer membrane more greatly than the inner membrane, whereas a longer-chain alkanol of pentanol at a concentration of 1.125% damaged both of the outer and inner membranes. Then we investigated the envelope stress response to <em>n</em>-alkanols by σ<sup>E </sup>factor by ratiometric analysis of <em>rpoE</em> promoter activity for the downstream GFPuv expression referenced to that of housekeeping sigma 70 (σ<sup>70 </sup>) recognizing <em>lacUV5</em> promoter for red fluorescent protein (RFP) expression. The results indicated that the relative activity of <em>rpoE</em> promoter by pentanol was much greater than that of ethanol. The degree of its sensitization by <em>rpoE</em> deficiency was much more remarkable for cells treated with pentanol than for those with ethanol. The results suggest that the response of the σ<sup>E</sup> plays a significant role in the membrane integrity and survival of <em>E. coli </em>cells treated with n-alkanols depending on the alkyl chain length of the molecule.</p>}, number={2}, journal={Academia Journal of Biology}, author={Bui, Huong Thi}, year={2022}, month={Jun.}, pages={91–104} }