TY - JOUR AU - Thanh Hang, Nguyen Thi AU - Phuong Hoang, Nguyen Thi AU - Van Khiem, Dinh AU - Xuan Huyen, Phan PY - 2022/06/23 Y2 - 2024/03/29 TI - Study on in vitro propagation of \(\textit{Vaccinium myrtillus}\) Linn. via nodal culture method JF - Academia Journal of Biology JA - AJB VL - 44 IS - 2 SE - Articles DO - 10.15625/2615-9023/16546 UR - https://vjst.net/index.php/vjbio/article/view/16546 SP - 53-64 AB - <p><em>Vaccinium myrtillus</em> Linn. is an imported fruit tree with high economic value. Currently, in Vietnam there has not been any publication on <em>in vitro</em> propagation of the blueberry plant. This study is focused on <em>in vitro</em> propagation of <em>V. myrtillus</em> by nodal culture. The results showed that <em>in vitro</em> shoot growth and proliferation were the best when nodes were cultured on WPM medium supplemented with 0.5 mg/L zeatin, 30 g/L sucrose, 10 g/L agar, pH 5.8 (shoot height 4.24 cm, <br />1 shoot/explant, 8.60 nodes/shoot). The third nodes to sixth nodes proved to be suitable materials for <em>in vitro</em> propagation of <em>V. myrtillus</em>. The proliferation and growth of shoots on the medium without activated charcoal (plant height 3.92 cm, 1 shoot/explant, 8.60 nodes/shoot) were better than those grown on the medium supplemented with 1 g/L of activated charcoal (plant height 3.64 cm, 1 shoot/explant, 7.90 nodes/shoot). For the <em>in vitro</em> rooting stage, the WPM medium containing 1.0 mg/L IBA, 30 g/L sucrose, 10 g/L agar, pH 5.8 resulted in plant height 5.49 cm, root length 1.42 cm, root formation rate 100%. After transferring the cultivated-tissue Vaccinium myrtillus to the greenhouse, substrate with coconut fibre powder, the results were observed to be plant height 6.94 cm, root length 3.18 cm, survival rate 95%. The plantlet growth increased overtime after 4 months in the greenhouse.</p> ER -