Extractive spectrophotometric methods for determination of ciprofloxacin in pharmaceutical formulations using sulfonephthalein acid dyes


Authors

  • Nguyen Trung Dung
  • Le Hoc Bau
  • Le Quang Thao
  • Nguyen Dang Dat
DOI: https://doi.org/10.15625/2525-2321.2017-005341

Keywords:

Ciprofloxacin, extraction-spectrophotometry, ion pair complex, sulfonephthalein dyes

Abstract

Three simple, rapid, sensitive and accurate extractive-spectrophotometric method for the determination of ciprofloxacin in pharmaceutical preparation has been developed. These methods are based on the formation of yellow ion-pair complexes between the examined drug and three sulfonephthalein  acid dyes, namely; bromophenol blue (BPB), bromocresol green (BCG),  and bromothymol blue (BTB) in  acidic medium. The formed complexes were extracted with chloroform and measured at 420, the colored chromogen was stable for twenty four hours. The effect of optimum conditions via pH, dye concentration, time and solvent are studied. Beer’s law is obeyed in the concentration ranges 0.50-25.0 μg/mL with  molar absorptivity of 1.46´104, 1.83´104 and 2.07´104 L. mol-1. cm-1 and limit of detection (LOD) of 0.105, 0.101, 0.084 for BPB, BCG and BTB methods, respectively. No interference was observed from common excipients present in pharmaceutical formulations. The proposed method has been applied successfully to determine ciprofloxacin in pharmaceutical preparation (tablets, infusion and eye drops).

Keywords. Ciprofloxacin, extraction-spectrophotometry, ion pair complex; sulfonephthalein  dyes.

References

Sharma PC, Jain A, Jain S, Pahwa R, Yar MS. Ciprofloxacin: review on developments in synthetic, analytical, and medicinal aspects, J. Enzyme Inhib Med Chem, 25(4), 577-89 (2010).

Janis Vella, Francesca Busuttil et al. A simple HPLC–UV method for the determination of ciprofloxacin in human plasma, Journal of Chromatography B, 989(1), 80-85 (2015).

3.O. Szerkus, J. Jacyna, A. Gibas, M. Sieczkowski, D. Siluka, M. Matuszewski, R. Kaliszan, M.J. Markuszewski. Robust HPLC–MS/MS method for levofloxacin and ciprofloxacin determination in human prostate tissue, Journal of Pharmaceutical and Biomedical Analysis, 132(5), 173-183 (2017).

Kuldeep Kaur, Ashwini Kumar, Ashok Kumar Malik, Baldev Singh, and A. L. J. Rao. Spectrophotometric Methods for the Determination of Fluoroquinolones: A Review, Critical Reviews in Analytical Chemistry, 38, 2-18 (2008).

M. Rizk, F. Belal, F. Ibrahim, S. M. Ahmed, and N. M. El-Enany. A simple kinetic spectrophotometric method for the determination of certain 4-quinolones in drug formulations, Scientia Pharmaceutica, 68(1), 173-188 (2000).

Nagaralli, B.S, Seetharamappa, J, Melwanki, M. B. Sensitive spectrophotometric methods for the determination of amoxicillin, ciprofloxacin and piroxicam in pure and pharmaceutical formulations, J. Pharm. Biomed. Anal, 29(5), 859-864 (2002).

Mostafa S, El-Sadek M, Alla E. A. Spectrophotometric determination of ciprofloxacin, enrofloxacin and pefloxacin through charge transfer complex formation, J. Pharm. Biomed. Anal, 27(1-2), 133-142 (2002).

Amina Mohamed El-Brashy, M. E.-S. M., and Fawzi Abdallah El-Sepai. Spectrophotometric Determination of Some Fluoroquinolone Antibacterials through Charge-transfer and Ion-pair Complexation Reactions. Bull. Korean Chem. Soc., 25 (3), 365-372 (2004).

Alaa S. Amin. Quantitation of Some Recently Introduced Antibacterial Drugs Using Sudan III as Chromogenic Reagent, Microchimica Acta, 134(1), 89-94 (2000).

El-Brashy A. M, Metwally M. E, El-Sepai F.A. Spectrophotometric determination of some fluoroquinolone antibacterials by binary complex formation with xanthene dyes, II Farmaco, 59(10), 809-817(2004).

Tadesse Haile Fereja, Muluneh Fromsa, Tizita Yirga Mola. UV-Visible Spectrophotometric Method Development and Quantification of Ciprofloxaciline in Tablets Dosage Form, American Journal of Pharmacy and Pharmacology, 2(1), 1-8(2015).

Abdel-Ghani NT, Rizk MS, Mostafa M. Extractive determination of ephedrine hydrochloride and bromhexine hydrochloride in pure solutions, pharmaceutical dosage form and urine samples, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 111, 131-41 (2013).

Ali E. A, Adawy A. M., El-Shahat M. F., Amin A. S. Simple spectrophotometric methods for determination of fluoxetine and clomipramine hydrochlorides in dosage forms and in some post-mortem biological fluids samples, Egyptian Journal of Forensic Sciences, 6(4), 370-380 (2016).

ICH Topic Q2 (R1). Validation of analytical procedures: text and methodology (CPMP/ICH/281/95); accessed June 30 (2010).

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Published

31-12-2017

How to Cite

Dung, N. T., Bau, L. H., Thao, L. Q., & Dat, N. D. (2017). Extractive spectrophotometric methods for determination of ciprofloxacin in pharmaceutical formulations using sulfonephthalein acid dyes. Vietnam Journal of Chemistry, 55(6), 767. https://doi.org/10.15625/2525-2321.2017-005341

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