ISOLATION AND IDENTIFICATION OF BIOFLOCCULANT-PRODUCING BACTERIA FROM CASSAVA CULTIVATED SOIL OF VIET NAM
Keywords:Bioflocculant producing-bacteria, flocculating rate, IAA, nitrogen-fixing bacteria, Bacillus aryabahattai, Enterobacter sp., Bacillus subtilis.
AbstractMicroorganisms are found everywhere in the environment and play a leading role in countless natural processes, and amongst them, soil microorganisms play important roles in plant performance by improving mineral nutrition. Soil bacteria possess different biological activities; for example, producing bioactive compounds such as bioflocculant which makes soil smoother and more breathable, as plant growth stimulants or compounds with antibiotic activity. Beside this, the same soil bacteria could also be able to fix atmospheric nitrogen, converting it into the form that plants can use. In order to make a microbial formula from local bacteria capable of producing bioflocculant as well as IAA and fixing nitrogen towards microbial organic fertilizer production for application in cassava cultivation, the bacteria were isolated from cassava cultivated soils and screened for above mention activities. From 37 cassava root and cassava cultivated soil samples collected at different provinces in the North, Central and South regions of Vietnam, 80 strains of bacteria with bioflocculant activity over 50% were screened. In which 3 strains (ĐQT1 Po6, ĐTAN6 Po2 and ĐSBV Po1) possess not only high bioflocculant and IAA producing capacities, but also the ability to fix atmospheric nitrogen. The flocculant rate of these strains was 80÷90% and can produce 6÷11 µg/mL IAA. The selective strains were identified by having an analysis of 16S rRNA gene sequence. The obtained results showed that the 16S rRNA sequence of ĐQT1 Po6 was 100% identifiable to corresponding sequence of Bacillus aryabhattai FORT 21 (Ass.No MG561348.1); and the 16S rRNA sequences of ĐTAN6 Po2 and ĐSBV Po1 had 100% homology with those of Enterobacter sp. (Ass.No KJ184880.1) and Bacillus subtilis SAN1.5 (Ass.No KX098457.1), respectively. These strains are potential candidates as perfect inoculant for biofertilizer.
Aljuboori A.H., Uemura Y., Osman N.B., Yusup S. - Production of a bioflocculant from Aspergillus niger using palm oil mill effluent as carbon source, Bioresour. Technol, 171 (2014) 66-70. doi: 10.1016/j.biortech.2014.08.038.
Anthony U., Sekelwa C., Leonard M., Olubukola O. B., Farhad A., Anthony O. - Thermostable Bacterial Bioflocculant Produced by Cobetia spp. Isolated from Algoa Bay (South Africa), Int J Environ Res Public Health, 9(6) (2012) 2108-2120. doi: 10.3390/ijerph9062108.
Bhattacharyya C., Bakshi U., Mallick I., Mukherji S., Bera B. and Ghosh A. - Genome-Guided Insights into the Plant Growth Promotion Capabilities of the Physiologically Versatile Bacillus aryabhattai Strain AB211, Front. Microbiol, 8 (2017) 411. doi:10.3389/fmicb.2017.00411.
Borgio J.F., Bency B.J., Ramesh S., and Amuthan M. - Exopolysaccharide production by Bacillus subtilis NCIM 2063, Pseudomonas aeruginosa NCIM 2862 and Streptococcus mutans MTCC 1943 using batch culture in different media, Afr. J. Biotechnol, 9 (2009) 5454-5457. doi: 10.4314/ajb.v8i20.65989.
Bragadeeswaran S., Jeevapriya R., Prabhu K., Sophia Rani S., Priyadharsini S. and Balasubramanian T. - Exopolysaccharide production by Bacillus cereus GU812900, a fouling marine bacterium, African Journal of Microbiology Research, 5(24) (2011) 4124-4132. doi: 10.5897/AJMR11.375.
Cao Ngoc Diep & Pham Si Phuc - Isolation and identification of bacteria producing bio-flocculants in leachate and application in leachate treatment, Can Tho University Journal of Science, 28 (2013) 86-92 (in Vietnamese).
Costa O. Y. A., Raaijmakers J. M., Kuramae E.E. - Microbial Extracellular Polymeric Substances: Ecological Function and Impact on Soil Aggregation, Front. Microbiol., (2018). doi.org/10.3389/fmicb.2018.01636.
Deng S. B., Bai R. B., Hu X. M., Luo Q. - Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment, Appl Microbiol Biotechnol, 60 (2003) 588-593. doi: 10.1007/s00253-002-1159-5.
Deng S. B., Bai R. and Hu X. - Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment, Appl. Microbial Biotechno, 60 (2002) 588-593.
Deng S., Yu G., Ting Y. - Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal, Colloids and Surfaces B: Biointerfaces, 44 (2005) 179-186. doi.org/10.1016/j. colsurfb.2005.06.011.
Dung L. N., Quyen Đ. N and Ty V. P. - Microbiology, Viet Nam education publishing house limited Company, 2010 (in Vietnamese).
Gao J., Bao Y. H., Xin X. M., Liu X. Y., Li Q. and Zhang F. Y. - Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31, J Zhejiang Univ Sci B, 7(3) (2006) 186-192. doi: 10.1631/jzus.2006.B0186.
Gouveia G.J. , Silva S. L. A., Santos L. C. E., Martins S. E. and López Q. - Optimization of bioflocculant production by Bacillus spp. from sugarcane crop soil or from sludge of the agroindustrial effluent. Brazilian Journal of Chemical Engineering, 36 (02) (2019) 627 - 637. doi.org/10.1590/0104-6632.20190362s20180360.
Guo J.Y., Yang C.P., Peng L.Y. - Preparation and characteristics of bacterial polymer using pre-treated sludge from swine wastewater treatment plant. Bioresour, Technol, 152 (2014) 490-498. doi: 10.1016/j.biortech.2013.11.037.
Hasuty A., Choliq A. and Hidayat I. - Production of Indole Acetic Acid (IAA) by Serratia marcescens subsp. marcescens and Rhodococcus aff. qingshengii, International Journal of Agricultural Technology, 14(3) 2018 299-312.
Huynh Van Tien, Cao Ngoc Diep and Truong Trong Ngon. - Optimization of bioflocculants synthesis of Bacillus aryabhattai KG12S and testing of biogas wastewater treatment from swine farms, Can Tho University Journal of Science, Part B: Agriculture, Fisheries and Biotechnology, 37(1) (2015) 32-41 (in Vietnamese).
Jeganathan A., Ramamoothy S. and Thillaichidambaram M. - Optimization of extracellular polysaccharide production in Halobacillus trueperi AJSK using response surface methodology, African Journal of Biotechnology, 13(48) (2014) 4449-4457. doi: 10.5897/AJB2014.14109.
Jie G., Hua-ying B., Ming-Xiu X., Yuan-xia L., Qian L., Yan Z. - Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31, J Zheijiang Univ Sci, 7 (2006) 186-192. http://dx.doi.org/10.1631/jzus.2006.B0186.
Kolwzan B., Adamiak W., Grabas K., Pawelczyk A. - Introduction to Environmental microbiology, 2006,112 pages. http://www.oficyna.pwr.wroc.pl.
Kumar B. L. and Gopal D. V. R. S. - Effective role of indigenous microorganisms for sustainable environment, 3 Biotech, 5 (2015) 867–876. doi.org/10.1007/s13205-015-0293-6.
Li H., Wu S., Du C., Zhong Y. and Yang C. - Preparation, Performances, and Mechanisms of Microbial Flocculants for Wastewater Treatment, Int J Environ Res Public Health, 17(4), 2020 1360. doi: 10.3390/ijerph17041360.
Li Q., Liu H.L., Qi Q., Wang F., Zhang Y. - Isolation and characterization of temperature and alkaline stable bioflocculant from Agrobacterium. sp. M503. New Biotechnology, (2010) 789-794. doi.org/10.1016/j.nbt.2010.09.002.
Makapela B., Okaiyeto K., Ntozonke N., Nwodo U., Green E., Mabinya L., Okoh A. - Assessment of Bacillus pumilus isolated from Fresh Water Milieu for bioflocculant production, Applied Sciences, 6 (2016) 211-231. doi. org/10.3390/app6080211.
Muhammadi & Muhammad Afzal. - Optimization of water absorbing exopolysaccharide production on local cheap substrates by Bacillus strain CMG1403 using one variable at a time approach, Journal of Microbiology, 52 (2014) 44-52. doi.org/10.1007/s12275-014-2622-6.
Okaiyeto K., Nwodo, U., Mabinya, L., Okoli, A., Okoh A. - Evaluation of flocculating performance of a thermostable bioflocculant produced by marine Bacillus sp, Environonmental Technology, 37 (2016) 1829-1842. doi.org/10.1080/095 93330.2015.1133717.
Park M., Kim C., Yang J., Lee H., Shin W., Kim S., Sa T. - Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea, Microbiological Research, 160(2), 2005 127-133. doi.org/10.1016/j.micres.2004.10.003.
Patten C.L. and Glick B.R. - Role of Pseudomonas putida indole acetic acid in development of the host plant root system, Appl environ microbiol, 68 (2002) 3795-3801.
Porwal H.J., Mane A.V., Velhal S.G. - Biodegradation of dairy effluent by using microbial isolates obtained from activated sludge, Water Resour. Ind, 9 (2015) 1-15. doi: 10.1016/j.wri.2014.11.002.
Prasertsan P., Dermlim W., Doelle H., Kennedy J. - Screening, characterization and flocculating property of carbohydrate polymer from newly isolated Enterobacter cloacae WD7, Carbohydrate Polymers, 66 (2006) 289-297. doi. org/10.1016/j.carbpol.2006.03.011.
Ratto M., Suihko L. Siika-aho M. - Polysaccharide-producing bacteria isolated from paper machine slime deposits, J Ind Microbiol Biotechnol, 32 (2005) 109-114. doi 10.1007/s10295-005-0210-9
Rebah F. B., Mnif W., and Siddeeg S. M. - Microbial Flocculants as an Alternative to Synthetic Polymers for Wastewater Treatment: A Review, Symmetry, 10 (2018) 556. doi:10.3390/sym10110556.
Salehizadeh H. and Shojaosadati S.A. - Isolation and characterization of a bioflocculant produced by Bacillus furmus, Biotech. Letters, 24 (2002) 35-40. doi: 10.1023/A:1013853115624.
Salehizadeh H., Shojaosadati S. - Extracellular biopolymeric flocculants: Recent trends and biotechnology importance, Biotechnology Advances, 19 (2001) 371-385. doi. org/10.1016/S0734-9750(01)00071-4.
Sekelwa C., Anthony U., Vuyani M., Anthony O. - Characterization of a thermostable polysaccharide bioflocculant produced by Virgibacillus species isolated from Algoa bay, African Journal of Microbiology Resource, 7 (2013) 2925-2938. doi.org/10.5897/AJMR12.2371.
Sharon J. A., Hathwaik L.T., Glenn G.M., Imam S. H., and Lee C.C. - Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth, Journal of Soil Science and Plant Nutrition, 16(2) 2016 525 - 536.
Sirajunnisa A. R., Vijayagopal V., Viruthagiri T. - Influence of various parameters on exopolysaccharide production from Bacillus sibtilis, International Journal of ChemTech Research, 5(5) (2013) 2221-2228.
Suryani, Ambarsari L., Artika I. A., Susanti H.E. - Characterization of Bioflocculant Producing-Bacteria Isolated from Tapioca Waste Water, HAYATI Journal of Biosciences, 18(4) (2011) 193-196. doi.org/10.4308/hjb.18.4.193.
Tram T. T. Đ., Xuyen K. C. and Son B. H. - Isolation, selection and identification of soil bacteria in the root zone stimulating plant growth in pepper sparrow (Piper nigrum L.) grown in Chon Thanh and Loc Ninh Districts of Binh Phuoc provice, Summary report on student scientific research, Saigon University., Ho Chi Minh City, 2016, pp. 32-34 (in Vietnamese).
Tzollas N. M., Zachariadis G. A., Aristidis N. A. & John A. S. - A new approach to indophenol blue method for determination of ammonium in geothermal waters with high mineral content, International Journal of Environmental Analytical Chemistry, 90 (2) 2010 115-126, doi: 10.1080/03067310902962528.
Vardharajula S., and Ali S. Z. - The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation, Microbiology, 84 (2015) 512-519. doi: 10.1134/s0026261715040153.
Wang L., Ma F., Qu Y., Sun D., Li A., Guo J., Yu B. Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaericus F6, World Journal of Microbiology and Biotechnology, 27 (2011) 2559-2565. doi.org/10.1007/s11274-011-0726-2.
Wang X., Sharp C. E., Jones G. M., Grasby S. E., Brady A. L., Dunfield P. F. - Stable-isotope probing identifies uncultured planctomycetes as primary degraders of a complex heteropolysaccharide in soil, Appl. Environ. Microbiol, 81 (2015) 4607-4615. doi: 10.1128/aem.00055-15.
Xia S., Zhang Z., Wang X., Yang A., Cheng L., Zhao J., Leonard D., Jaffrezic-Renault N. - Production and characterization of bioflocculant by Proteus mirabilis TJ-1, Bioresource Technology, 99 (2008) 6520-6527. doi.org/10.1016/j. biortech.2007.11.031.
Xiong Y., Wang Y., Yu Y., Li Q., Wang H., Cheng R., He N. - Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis, Applied and Environmental Microbiology, 76 (2010) 2778 2782. doi.org/10.1128/AEM.02558-09.
Zhang Z., Lin B., Xia S., Wang X., Yang A. - Production and application of a novel bioflocculant by multiple-microorganism consortia using brewery wastewater as carbon source, Journal of Environmental Sciences, 19 (2007) 667-673. doi.org/10.1016/S1001-0742(07)60112-0.
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