Optimisation of genetic transformation procedure for watermelon (Citrullus lanatus Thumb.)

Nguyen Thi Thanh Nga; Ho Manh Tuong; Pham Thi Van; Nguyen Tuong Van; Chu Hoang Ha; Le Tran Binh

doi:10.15625/0866-7160/v34n3.2473

Optimisation of genetic transformation procedure for watermelon (Citrullus lanatus Thumb.)

Authors

Nguyen Thi Thanh Nga VAST
Ho Manh Tuong Institute of Biotechnology, VAST
Pham Thi Van Institute of Biotechnology, VAST
Nguyen Tuong Van Institute of Biotechnology, VAST
Chu Hoang Ha Institute of Biotechnology, VAST
Le Tran Binh Institute of Biotechnology, VAST

DOI: https://doi.org/10.15625/0866-7160/v34n3.2473

Keywords:

Agrobacterium, Citrullus lanatus, gene gus, genetic transformation, kanamycin

Abstract

In this study, the binary vector pCB-gusplus carrying nptII and gus - intron genes as selectable and reportable markers was used to optimize Agrobacterium-mediated gene transformation protocol of four Vietnam watermelon lines. Agrobacterium tumefaciens CV58 habouring pCB-gusplus in the concentration of OD₆₀₀ 0.6-0.8 was infected into 5-7 day cotyledons for 30 minutes and co-cultured for 3 days. The transformed leave materials was cultured in the selectable medium containing MS salts and vitamins supplemented with 1.5 mg/l BAP, 0.5 mg/l IBA, 200 mg/l kanamycin và 500 mg/l cefotaxime. Regenerated shoots was rooted in MS medium added with 0.2 mg/l IBA, 100 mg/l kanamycin and 250 mg/l cefotaxime There was a significant difference in the transformation frequency among watermelon lines. F1 TG939 line showed the transformation frequency of 1.87%), following F9 Tieulong - Thanglong of 1.85%, F1 Kinhkong and F9 Binhthuan lines showed the transformation frequyency of 0%. The analysis of the obtained lines using GUS histochemical assay and PCR reaction with specific primers of ntpII gene had confirmed the presence of gus gene into 5 transgenic lines.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

PDF (Tiếng Việt)

Abstract View: 304
PDF Downloaded: 519

Published

03-10-2012

How to Cite

Thanh Nga, N. T., Tuong, H. M., Van, P. T., Van, N. T., Ha, C. H., & Binh, L. T. (2012). Optimisation of genetic transformation procedure for watermelon (Citrullus lanatus Thumb.). Academia Journal of Biology, 34(3), 389–396. https://doi.org/10.15625/0866-7160/v34n3.2473

Download Citation

Issue

Vol. 34 No. 3 (2012)

Section

Articles

Most read articles by the same author(s)

Nguyen Thi Tam, Nguyen Phu Tam, Vu Thi Hanh Nguyen, Nguyen Khac Hung, Chu Nhat Huy, Pham Bich Ngoc, Chu Hoang Ha, Phi Quyet Tien, Isolation and screening of endophytic bacteria from Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv) for biosynthesis β-glucosidase , Academia Journal of Biology: Vol. 40 No. 2 (2018)
Tran Thi Hong Thuy, Do Thi Gam, Nguyen Khac Hung, Pham Bich Ngoc, Chu Hoang Ha, In vitro micropropagation of an endangered medicinal orchild (Anoectochilus setaceus Blume) through protocorm-like bodies , Academia Journal of Biology: Vol. 37 No. 1 (2015)
Nguyen Thi Mai, Phan Thanh Binh, Do Thi Gam, Phan Hong Khoi, Nguyen Khac Hung, Pham Bich Ngoc, Chu Hoang Ha, Ha Thi Thanh Binh, Effects of light emitting diodes - led on regeneration ability of Coffea canephora mediated via somatic embryogenesis , Academia Journal of Biology: Vol. 38 No. 2 (2016)
Le Thi Hien, Nguyen Tuong Van, Effects of nano copper used in seed treatment for germination, growth, and productivity of maize , Academia Journal of Biology: Vol. 40 No. 4 (2018)
Hoang Thi Lan Anh, Ngo Thi Hoai Thu, Tran Huy Hoang, ZhiGang Zhou, Tran Que, Chu Hoang Ha, Truong Nam Hai, Dang Diem Hong, Karyotype analysis of Schizochytrium mangrovei PQ6 using dapi (4, 6-diamidino-2-phenylidole) counterstaining and pulsed-field gel electrophoresis (pfge) techniques , Academia Journal of Biology: Vol. 37 No. 1 (2015)
Nguyen Khac Hung, Pham Bich Ngoc, Nguyen Thi Thu Hien, Nguyen Thi Thuy Huong, Do Thi Gam, Le Duy Hung, Chu Hoang Ha, Study on the effects of led light on physiological and morphological characteristics of dang shen (Codonopsis sp.) growth in in vitro condition , Academia Journal of Biology: Vol. 38 No. 2 (2016)
Pham Thi Thanh Nhan, Nguyen Huy Cuong, Le Tran Binh, Extracting and analysising anthocyanin content from different plants , Academia Journal of Biology: Vol. 33 No. 4 (2011)
Tran My Linh, Vu Huong Giang, Le Quynh Lien, Nguyen Tuong Van, Ninh Khac Ban, Chau Van Minh, Antibacterial activity of some mangrove species in Xuan Thuy national park, Nam Dinh, Vietnam , Academia Journal of Biology: Vol. 35 No. 3 (2013)
Vu Thi Lan, Pham Bich Ngoc, Chu Hoang Ha, Le Tran Binh, Agrobacterium–mediated transformation of cry3ca1 gene into kb1 sweet potato cultivar , Academia Journal of Biology: Vol. 40 No. 2 (2018)
Hoang Dang Hieu, Chu Thi Thu Ha, Pham Bich Ngoc, Lam Dai Nhan, Nguyen Thi Thuy Huong, Chu Hoang Ha, Study on genetic diversity in Morinda officinalis F. C. How populations in Quang Ninh using issr marker , Academia Journal of Biology: Vol. 38 No. 1 (2016)

1 2 3 4 5 > >>